Composite

Part:BBa_K3624007

Designed by: Emily Hagens   Group: iGEM20_Lethbridge   (2020-10-26)


Tu-AMP2: Antifungal peptide.

Tu-AMP2 is a thionin-like, 20 amino acids long antimicrobial peptide. It is effective against F. Oxysporum and able to bind chitin in a reversible way. This composite part includes the Ptac-M3 promoter (BBa_K3254017), RBS BBa_B0034, Tu-AMP2 coding region BBa_K3624006, and double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 85
    Illegal XbaI site found at 100
    Illegal SpeI site found at 172
    Illegal PstI site found at 186
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 85
    Illegal SpeI site found at 172
    Illegal PstI site found at 186
    Illegal NotI site found at 91
    Illegal NotI site found at 179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 85
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 85
    Illegal suffix found in sequence at 172
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 85
    Illegal XbaI site found at 100
    Illegal SpeI site found at 172
    Illegal PstI site found at 186
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None